rabbit polyclonal anti rock2 antibody  (Proteintech)

Journal: iScience

Article Title: ZXDC enhances cervical cancer metastasis through IGF2BP3 -mediated activation of RhoA / ROCK signaling

doi: 10.1016/j.isci.2023.107447

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti- ROCK2 , Cell Signaling Technology , Cat#8236, RRID: AB_10829468.

Techniques: Virus, Recombinant, Reporter Assay, Chromatin Immunoprecipitation, Sequencing, Gene Expression, shRNA, Software

Journal: PLoS ONE

Article Title: Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells

doi: 10.1371/journal.pone.0226406

Figure Lengend Snippet: (A) Representative western blots showing the effects of ROCK1 or ZIPK knockdown on ROCK1 and ZIPK expression in CASMC and UASMC. (B) Quantification of ROCK1, ROCK2 and ZIPK levels in ROCK1 siRNA-treated cells compared to controls. (C) Quantification of ROCK1 and ZIPK levels in ZIPK siRNA-treated cells compared to controls. Statistical analysis was carried out with Dunnett’s post hoc test.

Article Snippet: The following primary antibodies were used in this study: rabbit monoclonal anti-ZIPK (1:1,000 dilution; Epitomics, Burlingame, CA; cat. no. 2568–1), rabbit polyclonal anti-ROCK1 (1:1,000 dilution; Epitomics; cat. no. S2711), rabbit polyclonal anti-ROCK2 (1:2,000 dilution; AbFrontier, Seoul, South Korea; cat. no. LF-PA0049), rabbit polyclonal anti-pT18/pS19-myosin light chain 2 (1:500 dilution; Cell Signaling; cat. no. 3674), rabbit anti-GAPDH (1:5,000 dilution; Santa Cruz Biotechnology; cat. no. sc-32233), goat polyclonal anti-SM22α (1:2,500 dilution; Novus Biologicals, Centennial, CO; cat. no. NB600-507), rabbit polyclonal anti-LC 20 (1:2,000 dilution; Rockland Immunochemicals, Limerick, PA; cat. no. 600-401-938), rabbit polyclonal anti-pT853-MYPT1 (1:1,000 dilution; EMD Millipore; cat. no. 36–003), rabbit polyclonal anti-pT696-MYPT1 (1:1,000 dilution; EMD Millipore; cat. no. ABS45), rabbit polyclonal anti-pT163-Par4 (1:1,000 dilution; Cell Signaling; cat. no. 2329), rabbit monoclonal anti-pS473-Akt (193H12) (1:1,000 dilution; Cell Signaling; cat. no. 4058); rabbit polyclonal anti-pT180/pY182-p38 MAP kinase (1:1,000 dilution; Cell Signaling; cat. no. 9211), rabbit monoclonal anti-pT202/Y204-ERK1/pT185/pY187-ERK2 (1:1,000 dilution; Cell Signaling; cat. no. 4370), rabbit anti-pS82-HSP27 (1:1,000 dilution; Cell Signaling; cat. no. 2401), and rabbit polyclonal anti-α-actin (1:1,000 dilution; Cytoskeleton, Inc.; cat. no. AAN01).

Techniques: Western Blot, Expressing

Journal: PLoS ONE

Article Title: Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells

doi: 10.1371/journal.pone.0226406

Figure Lengend Snippet: A schematic showing the main conclusions from this study regarding serum-induced phosphorylation of the regulatory light chains of myosin II (LC 20 ) and the regulatory kinases and phosphatase involved. Serum treatment of CASMC and UASMC activates Rho-associated coiled-coil kinase (ROCK) and zipper-interacting protein kinase (ZIPK) leading to phosphorylation of the regulatory myosin-targeting subunit (MYPT1) of myosin light chain phosphatase (MLCP) at T853 and prostate apoptosis response 4 (Par4) at T163, both of which result in inhibition of MLCP activity. ZIPK activation involves phosphorylation by ROCK and autophosphorylation. Activated ROCK and ZIPK directly phosphorylate LC 20 at T18 and S19.

Article Snippet: The following primary antibodies were used in this study: rabbit monoclonal anti-ZIPK (1:1,000 dilution; Epitomics, Burlingame, CA; cat. no. 2568–1), rabbit polyclonal anti-ROCK1 (1:1,000 dilution; Epitomics; cat. no. S2711), rabbit polyclonal anti-ROCK2 (1:2,000 dilution; AbFrontier, Seoul, South Korea; cat. no. LF-PA0049), rabbit polyclonal anti-pT18/pS19-myosin light chain 2 (1:500 dilution; Cell Signaling; cat. no. 3674), rabbit anti-GAPDH (1:5,000 dilution; Santa Cruz Biotechnology; cat. no. sc-32233), goat polyclonal anti-SM22α (1:2,500 dilution; Novus Biologicals, Centennial, CO; cat. no. NB600-507), rabbit polyclonal anti-LC 20 (1:2,000 dilution; Rockland Immunochemicals, Limerick, PA; cat. no. 600-401-938), rabbit polyclonal anti-pT853-MYPT1 (1:1,000 dilution; EMD Millipore; cat. no. 36–003), rabbit polyclonal anti-pT696-MYPT1 (1:1,000 dilution; EMD Millipore; cat. no. ABS45), rabbit polyclonal anti-pT163-Par4 (1:1,000 dilution; Cell Signaling; cat. no. 2329), rabbit monoclonal anti-pS473-Akt (193H12) (1:1,000 dilution; Cell Signaling; cat. no. 4058); rabbit polyclonal anti-pT180/pY182-p38 MAP kinase (1:1,000 dilution; Cell Signaling; cat. no. 9211), rabbit monoclonal anti-pT202/Y204-ERK1/pT185/pY187-ERK2 (1:1,000 dilution; Cell Signaling; cat. no. 4370), rabbit anti-pS82-HSP27 (1:1,000 dilution; Cell Signaling; cat. no. 2401), and rabbit polyclonal anti-α-actin (1:1,000 dilution; Cytoskeleton, Inc.; cat. no. AAN01).

Techniques: Inhibition, Activity Assay, Activation Assay

Journal: Neural Regeneration Research

Article Title: The Rho-associated kinase inhibitors Y27632 and fasudil promote microglial migration in the spinal cord via the ERK signaling pathway

doi: 10.4103/1673-5374.230294

Figure Lengend Snippet: Microglia expressed ROCK2, and the ROCK inhibitors were not cytotoxic to cultured spinal cord microglia at the concentrations used. (A1) CD11b + DAPI double staining; (A2) ROCK2 + DAPI double staining; (A3) CD11b + ROCK2 double staining. Scale bar: 25 μm. (B) Mean LDH release after 1, 3 and 6 hours of treatment with Y27632 (10 μM), fasudil (41 μM) or control (phosphate-buffered saline). (C) ROCK activity in microglia after Y27632 (10 μM) and fasudil (41 μM) treatment. Data are shown as the mean ± SEM ( n = 4; one-way analysis of variance followed by Tukey's post hoc test). ** P < 0.01. ROCK: Rho-associated kinase; LDH: lactate dehydrogenase; DAPI: 4′,6-diamidino-2-phenylindole.

Article Snippet: Cells were incubated overnight with rabbit anti-IBA1 polyclonal antibody (1:300; Wako, Richmond, VA, USA), mouse anti-CD11b monoclonal antibody (1:100; BD Biosciences, San Jose, CA, USA), rabbit anti-ROCK2 polyclonal antibody (1:300; Santa Cruz Biotechnology) or negative control (PBS) at 4°C.

Techniques: Cell Culture, Double Staining, Activity Assay